Biochemistry, Molecular Biology

Head of the technical facility:
William Cazenave
+33 (0)5-57-57-95-14

The Biochemistry and Molecular Biology technical facility is located in room S 2113 on the ground floor of the BBS. The facility is divided into two sections: biochemistry and molecular biology. Some fifteen people work here on a regular basis.

The biochemistry department is the largest in terms of surface area, equipment and users.
The Western Blot technique, which enables the highly specific detection and identification of proteins in a sample, is used routinely. We perform protein extraction on various models and tissues, protein assay and migration, gel activation, membrane transfer, visualization of total proteins and proteins of interest, and sample quantification.
We also measure yeast growth under different culture conditions.
ELISA tests are also carried out on the technical platform. The principle of this technique is based on the visualization of an antigen-antibody reaction using an enzymatic colorimetric reaction.
The largest devices are the ChemiDoc MP (chemiluminescence revelation) and an odyssey (fluorescence revelation).

In molecular biology, we perform DNA and RNA extractions on various models (human cells, murine cells or yeast).
A Nanodrop is used to assay DNA, RNA and proteins. The platform is equipped with thermocyclers to perform PCR for gene cloning, sequencing of genetic variants from human, yeast or plasmid DNA, and site-directed mutagenesis on plasmid DNA.
The facility is also equipped with a real-time thermocycler for RT-PCR and quantitative PCR to study gene expression.